Propagation of almond by shoot tip culture.

High frequency in vitro propagation of Phyllanthus amarus Schum. & Thom. by shoot tip culture.

With the aim of micropropagation of Phyllanthus amarus, an important medicinal herb, shoot tips were cultured in Murashige and Skoog's medium supplemented with kinetin/ BAP singly or in combination with IAA. Growth regulators at lower range (0.1-1.0 mg L(-1)) stimulated direct regeneration of shoots. Kinetin was superior to BAP and kinetin-IAA combination was more suitable than kinetin alone. A...

A Micropropagation System for Cloning of Hemp (cannabis Sativa L.) by Shoot Tip Culture

This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·Lsucrose, 5.5 g·Lagar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·LTDZ, 0.1 mg·LNAA supported the maximal auxil...

Meristem-tip culture for propagation and virus elimination.

Improvement of shoot-tip culture proliferation in banana using PEG6000. Leila Saeedavi, Ali Soleimani* and Mohammad Esmaeil Amiri

In order to improve the shoot proliferation rate of ‘Dwarf Cavendish’ banana shoot tip cultures, polyethylene glycol (PEG6000) was employed at concentrations of 2, 4, and 6% (w/v). The medium without PEG was considered as negative control. An increasing of shoot proliferation was achieved in MS medium containing 2% PEG. Furthermore, application of 2% PEG significantly increased the shoot dry we...

In Vitro Plant Regeneration Using Shoot Tip Culture in Commercial Cultivar of Teak

A protocol for direct shoot regeneration without intervening callus phase was developed by shoot tip culture of MS (murashinge and skoog) medium for teak. The study reveal that aseptically inoculated shoot tip of teak in established shoot induction and shoot multiplication was also observed at same concentration .At BAP 1mg/l, IAA 1mg/l and kin 1mg/l the shoots were weak ,tiny and non separable...